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Caamano J , DiRado M , Iizasa T , Momiki S , Fernandes E , Ashendel C , Noda M , Klein-Szanto AJP
Partial suppression of tumorigenicity in a human lung cancer cell line transfected with Krev-1
Molecular Carcinogenesis. 1992 ;6(4) :252-259
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A human non-small-cell lung carcinoma cell line, Calu-6 (from an anaplastic carcinoma), was transfected with the Ki-ras-related anti-oncogene Krev-1. Several transfectant lines were obtained that showed a reduced tumorigenicity in nude mice with respect to the parental and control transfected cell lines. This decrease was approximately 50% in tumor incidence at 4 wk after subcutaneous inoculation of the transfected cells. In addition, the volume of the Calu-6 revertant-derived tumors was three to 10 times smaller than that of the equivalent tumors produced by inoculation of the control cell line transfected with the neomycin-resistant gene. Krev-1-transfected cells that exhibited reduced tumorigenicity expressed Krev-1 mRNA and had variable numbers of copies of the Krev-1 gene. Moreover, Krev-1-transfected cells exhibited a more differentiated squamous epithelial morphology than the parental and control cell lines did. Moderately elevated levels of protein kinase C activity were detected in some revertant clones. Such activity correlated with the level of expression of Krev-1 mRNA in most cases. In summary, Krev-1 induced important morphological and biological changes in transfected Calu-6 cells that we interpreted as partial reversion of the malignant phenotype.
08991987 (ISSN) Cited By: 5; Export Date: 31 May 2006; Source: Scopus CODEN: MOCAE Language of Original Document: English Correspondence Address: Klein-Szanto, A.J.P.; Fox Chase Cancer Center; 7701 Burholme Ave. Philadelphia, PA 19111, United States Chemicals/CAS: DNA, Neoplasm; Protein Kinase C, EC