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Nakajima PB , Di Vincenzo JP , Jameson SC , Gascoigne NRJ
Chromosome 14 in B10.A(18R) mice is recombinant and includes Tcra-Va alleles
Immunogenetics. 1992 ;35(3) :190-198
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Analysis of mouse Tcr genes has previously defined at least five different Tcra-V haplotypes among inbred strains of mice. For mice of the Tcra-Vb haplotype, including C57BL/10 (B10), T-cell expression of the Tcra-V11 gene subfamily can be detected with a monoclonal antibody, 1.F2. In the course of further characterizing the specificity of 1.F2, we found that it fails to recognize Tcra-V11-expressing T-cell hybrids derived from the B10 congenic strain, B10.A(18R)/SgIcr. Moreover, staining analysis indicated that the Va11 epitope recognized by 1.F2 is not expressed by peripheral T cells from several different B10.A(18R) colonies with the exception of that at the Research Institute of Scripps Clinic. Nucleotide sequences were determined for cDNA representing rearranged Tcra-V11 genes from two independent, B10.A(18R)/SgIcr derived T-cell hybrids. The two Tcra-V11 gene segments were identical and the predicted amino acid sequence differed by at least five residues from Tcra-V11 sequences previously obtained from B10.A mice. Southern blot analysis of restriction fragment length polymorphisms (RFLP) associated with Tcra-V11, as well as Tcra-V1, subfamily genes revealed that the B10.A(18R) mouse has inherited Tcra-Va alleles rather than the expected Tcra-Vb alleles from the B10 strain. RFLP analysis of the Rib-1 locus, located in close proximity to the Tcra locus on chromosome 14, showed that B10.A(18R) carries the Rib-1b allele from B10. These results indicate that the B10.A(18R) mouse has inherited a recombinant chromosome 14 with a recombination event having occurred between the Rib-1 locus and the Tcra-V gene subfamilies examined. Inheritance of Tcra-Va alleles in B10.A(18R) probably originated from strain 129/J which breeding records show was used in the first cross with B10.A in the production of B10.A(18R) and which we found exhibits Tcra-V11a RFLPs.
00937711 (ISSN) Cited By: 1; Export Date: 31 May 2006; Source: Scopus CODEN: IMNGB Language of Original Document: English Correspondence Address: Nakajima, P.B.; Department of Pathology; Fox Chase Cancer Center Philadelphia, PA 19111, United States Molecular Sequence Numbers: GENBANK: M55634, M55635, M62548, M62549, M62550, M62551, M62552, M62553, X59621, X59622; Chemicals/CAS: Antibodies, Monoclonal; Epitopes; Receptors, Antigen, T-Cell, alpha-beta