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Paine TM , Fontanini G , Basolo F , Geronimo I , Elliott JW , Russo J
Mutated c-Ha-ras oncogene alters cytokeratin expression in the human breast epithelial cell line MCF-10A
American Journal of Pathology. 1992 ;140(6) :1483-1488
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MCF-10A, a spontaneously immortalized human breast epithelial cell line, has been transformed by transfection with the mutated c-Ha-ras oncogene obtained from T24 carcinoma cells. The pattern of cytokeratin expression was studied in MCF-10A cells in comparison with plasmid transfected or MCF-10Aneo cells, normal ras proto oncogene transfected or MCF-10AneoN cells, and transformed or MCF-10AneoT cells. Cytokeratin expression was studied by western immunoblot of total cellular proteins separated by two-dimensional gel electrophoresis. Blots with cytokeratin specific AE1 and AE3 antibodies showed identical molecular weight species of cytokeratins in MCF-10A, MCF- 10Aneo, MCF-10AneoN, and MCF-10AneoT cells; however, in MCF-10AneoT cells, the intensity of immunostaining and the number of immunoreactive phosphorylated polypeptides keratins 7, 8, 15, and 16 was decreased. It was concluded that c-Ha-ras oncogene-induced transformation alters quantitatively the cytokeratin pattern of human breast epithelial cells and that these changes could explain some of the morphologic alterations observed in c-Ha- ras transformed cells.
00029440 (ISSN) Cited By: 6; Export Date: 31 May 2006; Source: Scopus CODEN: AJPAA Language of Original Document: English Correspondence Address: Russo, J.; Department of Pathology; Fox Chase Cancer Center; 7701 Burholme Avenue Philadelphia, PA 19111, United States Chemicals/CAS: Keratin, 68238-35-7