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Sonobe H , Takeuchi T , Liag SB , Taguchi T , Yuri K , Shimizu K , Iwata J , Furihata M , Ohtsuki Y , Testa JR
A new human synovial sarcoma cell line, HS-SY-3, with a truncated form of hybrid SYT/SSX1 gene
International Journal of Cancer. 1999 Jul 30;82(3) :459-464
AbstractRecent cytogenetical and molecular studies have indicated that synovial sarcoma harbors a t(X;18)(p11.2;q11.2) translocation, resulting in the formation of a hybrid SYT/SSX(SSX1 or SSX2) gene. We newly established a human cell line, HS-SY-3, from a synovial sarcoma. HS-SY-3 cells were shown to harbor the pathognomonic t(X;18)(p11.2;q11.2) translocation by chromosome analysis but not to exhibit the classical hybrid SYT/SSX transcripts induced by this translocation, using RT-PCR. To determine the reason for this discrepancy, we analyzed cDNA from HS-SY-3 cells, as well as the original sarcoma tissue by the rapid amplification of cDNA 3' end assay, and found that the chimaeric cDNA was 240 bp shorter than the previously established SYT/SSX1 cDNA due to truncation of the 3' side of SSX1. The HS-SY-3 cells should be useful for future functional studies of the SYT/SSX chimeric gene. Int. J. Cancer 82:459- 464, 1999. (C) 1999 Wiley-Liss, Inc.
NotesTimes Cited: 9 English Article 211YJ INT J CANCER