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Haddad A , Rose RW , Pohlschro?der M
The Haloferax volcanii FtsY homolog is critical for haloarchaeal growth but does not require the A domain
Journal of Bacteriology. 2005 ;187(12) :4015-4022
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Abstract
The targeting of many Sec substrates to the membrane-associated translocation pore requires the cytoplasmic signal recognition particle (SRP). In Eukarya and Bacteria it has been shown that membrane docking of the SRP-substrate complex occurs via the universally conserved SRP receptor (Sr?/? and FtsY, respectively). While much has been learned about the archaeal SRP in recent years, few studies have examined archaeal Sr?/FtsY homologs. In the present study the FtsY homolog of Haloferax volcanii was characterized in its native host. Disruption of the sole chromosomal copy of ftsY in H. volcanii was possible only under conditions where either the full-length haloarchaeal FtsY or an amino-terminally truncated version of this protein lacking the A domain, was expressed in trans. Subcellular fractionation analysis of H. volcanii ftsY deletion strains expressing either one of the complementing proteins revealed that in addition to a cytoplasmic pool, both proteins cofractionate with the haloarchaeal cytoplasmic membrane. Moreover, membrane localization of the universally conserved SRP subunit SRP54, the key binding partner of FtsY, was detected in both H. volcanii strains. These analyses suggest that the H. volcanii FtsY homolog plays a crucial role but does not require its A domain for haloarchaeal growth. Copyright © 2005, American Society for Microbiology. All Rights Reserved.
Notes
00219193 (ISSN) Cited By: 3; Export Date: 25 May 2006; Source: Scopus CODEN: JOBAA; DOI: 10.1128/JB.187.12.4015-4022.2005 Language of Original Document: English Correspondence Address: Pohlschro?der, M.; Department of Biology; University of Pennsylvania; 201 Leidy Laboratories; 415 South University Ave. Philadelphia, PA 19104, United States; email: pohlschr@sas.upenn.edu Molecular Sequence Numbers: GENBANK: AY187867; Chemicals/CAS: Bacterial Proteins; FtsY protein, Bacteria; Receptors, Cytoplasmic and Nuclear References: Allers, T., Ngo, H.P., Mevarech, M., Lloyd, R.G., Development of additional selectable markers for the halophilic archaeon Haloferax volcanii based on the leuB and trpA genes (2004) Appl. Environ. Microbiol., 70, pp. 943-953; Bitan-Banin, G., Ortenberg, R., Mevarech, M., Development of a gene knockout system for the halophilic archaeon Haloferax volcanii by use of the pyrE gene (2003) J. 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