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Vilgelm Anna , Lian Zenglin , Wang Hong , Beauparlant Stephen L , Klein-Szanto Andres , Ellenson Lora Hedrick , Di Cristofano Antonio
Akt-Mediated Phosphorylation and Activation of Estrogen Receptor a Is Required for Endometrial Neoplastic Transformation in Pten+/- Mice
Cancer Research. 2006 ;66(7) :3375-3380
PMID: AN 2006:307750   
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Abstract
PTEN is a tumor suppressor gene frequently mutated in human cancers. In vitro and in vivo studies have shown that PTEN can exert its tumor suppressive function through a variety of mechanisms, including regulation of cell death and cell proliferation. However, it is still unclear which of the many downstream pathways are crit. in each different tissue, in vivo. Loss of PTEN is the earliest detectable genetic lesion in the estrogen-related type I (endometrioid) endometrial cancer. Pten+/- mice develop endometrial neoplastic lesions with full penetrance, thus providing a model system to dissect the genetic and biochem. events leading to the transition from normal to hyperplastic and neoplastic endometrial epithelium. Here, we show that loss of Pten in the mouse endometrium activates Akt and results in increased phosphorylation of estrogen receptor a (ERa) on Ser167. ERa phosphorylation results, in turn, in the activation of this nuclear receptor both in vivo and in vitro, even in the absence of ligand, and in its increased ability to activate the transcription of several of its target genes. Strikingly, redn. of endometrial ERa levels and activity dramatically reduces the neoplastic effect of Pten loss in the endometrium, in contrast to complete estrogen depletion. Thus, we provide for the first time in vivo evidence supporting the hypothesis that loss of Pten and subsequent Akt activation result in the activation of ERa-dependent pathways that play a pivotal role in the neoplastic process. [on SciFinder (R)]
Notes
14 Mammalian Pathological Biochemistry Human Genetics Program,Fox Chase Cancer Center,Philadelphia,PA,USA. Journal 0008-5472 written in English.