FCCC LOGO Faculty Publications
Cairns Paul G
Methods for detecting hypermethylated promoters of tumor suppressor genes associated with ovarian cancer using methylation-specific PCR
. 2006 9/14/2005 :28 pp
PMID: AN 2006:272859   
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Abstract
Methods for the detecting hypermethylated promoters of tumor suppressor genes assocd. with ovarian cancer using methylation-specific PCR are provided. Several cancer genes including BRCA1, RASSF1A, APC, p14ARF, CDKN2A and DAP-kinase have hypermethylated CpG islands within the promoter region which are normally unmethylated. Hypermethylation can be identified by the sensitive methylation-specific PCR method which can identify 1 methylated allele in 1000 unmethylated alleles in body fluids. [on SciFinder (R)]
Notes
3-1 Biochemical Genetics Patent written in English. 20060323 169150-71-4 (dap kinase) Role: BSU (Biological study, unclassified), BIOL (Biological study) (gene for; methods for detecting hypermethylated promoters of tumor suppressor genes assocd. with ovarian cancer using methylation-specific PCR); 7631-90-5 (sodium bisulfite) Role: ARG (Analytical reagent use), DGN (Diagnostic use), ANST (Analytical study), BIOL (Biological study), USES (Uses) (methods for detecting hypermethylated promoters of tumor suppressor genes assocd. with ovarian cancer using methylation-specific PCR) A2 US 2004-610041 20040915