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Balogh Gabriela A , Heulings Rebecca , Mailo Daniel A , Russo Patricia A , Sheriff Fathima , Russo Irma H , Moral Raquel , Russo Jose
Genomic signature induced by pregnancy in the human breast
International Journal of Oncology. 2006 ;28(2) :399-410
PMID: AN 2006:150387   
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Abstract
We have postulated that the lifetime protective effect of an early pregnancy against breast cancer is due to the complete differentiation of the mammary gland characterized by a specific genomic signature imprinted by the physiol. process of pregnancy. For demonstrating this hypothesis we compared the genomic profile of the epithelium and the stroma of normal breast tissues from redn. mammoplasties performed in postmenopausal parous and nulliparous women. The epithelium and the stroma were sep. dissected using laser capture microdissection (LCM) and the RNA of each compartment and each sample was isolated, amplified using PCR methodol., and hybridized to cDNA glass-microarrays contg. 40,000 human cDNA features. The sepn. of the epithelial compartment from the interlobular stroma of Lob 1 using LCM allowed us to det. that the epithelial component contained 4,828 genes that were equally expressed in both nulliparous and parous women. There were 73 known genes that included immune-modulation-, DNA repair-, programmed cell death-, chromatin remodeling- and transcription-related genes, whereas in the breast of nulliparous women there were 20 different known genes that were upregulated. Our data provide evidence that breast tissues of postmenopausal parous women express in both the epithelial and the stromal compartments numerous genes that differ significantly from those present in breast tissues of postmenopausal nulliparous women, which could be important contributors to the genomic signature induced by an early full term pregnancy. [on SciFinder (R)]
Notes
14 Mammalian Pathological Biochemistry Fox Chase Cancer Center,Philadelphia,PA,USA. Journal 1019-6439 written in English.