FCCC LOGO Faculty Publications
Michie AM , Trop S , Wiest DL , Zuniga-Pflucker JC
Extracellular signal-regulated kinase (ERK) activation by the pre-T cell receptor in developing thymocytes in vivo
Journal of Experimental Medicine. 1999 Dec 6;190(11) :1647-1655
PMID: ISI:000084137800010   
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The first checkpoint in T cell development occurs between the CD4(-)CD8(-) and CD4(+)CD8(+) stages and is associated with formation of the pre-T cell receptor (TCR). The signaling mechanisms that drive this progression remain largely unknown. Here, we show that extracellular signal-regulated kinases (ERKs)-1/2 are activated upon engagement of the pre-TCR. Using a novel experimental system, we demonstrate that expression of the pre-TCR by developing thymocytes induces ERK-1/2 activation within the thymus. In addition, the activation of this pre-TCR signaling cascade is mediated through Lck. These findings directly link pre-TCR complex formation with specific downstream signaling components in vivo.
Times Cited: 15 English Article 263QY J EXP MED