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Bosma Gayle C , Oshinsky Jennifer , Kiefer Kerstin , Nakajima Pamela B , Charan Deepshika , Congelton Cecil , Radic Marko , Bosma Melvin J
Development of Functional B Cells in a Line of SCID Mice with Transgenes Coding for Anti-Double-Stranded DNA Antibody
Journal of Immunology. 2006 ;176(2) :889-898
PMID: AN 2006:12507
AbstractDeletion or inactivation of anti-self (DNA) B cells has been reported in non-autoimmune mice bearing Ig transgenes that code for Abs with specificity for dsDNA or ssDNA. However, we report a case in which anti-dsDNA B cells appear to escape both deletion and inactivation. We show that B cells (B220+IgM+) can develop in non-autoimmune SCID mice bearing two site-directed transgenes, 3H9(56R) and Vk8, that together code for an anti-dsDNA Ab. The B cells appear inactive, because the mice (56RVk8 SCID mice) generally lack serum Ig. However, 56RVk8 SCID mice are able to produce IgG Ab with specificity for dsDNA when they become leaky for T cells or are reconstituted with exogenous T cells from B cell-deficient JH-/- donors. Thus, anti-dsDNA B cells that escape deletion in 56RVk8 SCID mice appear fully functional and can differentiate, class switch, and give rise to IgG-producing cells in the presence of T cells and self-Ag. [on SciFinder (R)]
Notes15 Immunochemistry Institute for Cancer Research,Fox Chase Cancer Center,Philadelphia,PA,USA. Journal 0022-1767 written in English.