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Khazak V , Golemis EA , Weber L
Development of a yeast two-hybrid screen for selection of human Ras-Raf protein interaction inhibitors
Methods Mol Biol. 2005 ;310 :253-71
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A yeast two-hybrid screening system was developed to screen for small molecules that inhibit the interaction of the Ras and the Raf proteins. Hyperpermeable yeast strains useful for high-throughput screening (HTS) for the two-hybrid system were created. Differential inhibition of the Ras-Raf vs the hsRPB4-hsRPB7 interaction allowed the identification of selective inhibitors.
1064-3745 (Print) Journal Article