This is an archive of papers published by the staff and faculty of Fox Chase Cancer Center. For questions about content, please contact Talbot Research Library
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Khazak V , Golemis EA , Weber L
Development of a yeast two-hybrid screen for selection of human Ras-Raf protein interaction inhibitors
Methods Mol Biol. 2005 ;310 :253-71
PMID: 16353379 URL: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16353379
AbstractA yeast two-hybrid screening system was developed to screen for small molecules that inhibit the interaction of the Ras and the Raf proteins. Hyperpermeable yeast strains useful for high-throughput screening (HTS) for the two-hybrid system were created. Differential inhibition of the Ras-Raf vs the hsRPB4-hsRPB7 interaction allowed the identification of selective inhibitors.
Notes1064-3745 (Print) Journal Article