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Fashena SJ , Serebriiskii I , Golemis EA
The continued evolution of two-hybrid screening approaches in yeast: how to outwit different preys with different baits
Gene. 2000 May 30;250(1-2) :1-14
PMID: 10854774 URL: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=10854774
AbstractThe original two-hybrid system, an experimental approach designed to detect protein interactions, exploited the modular nature of many transcription factors. It has provided the intellectual and technical seed for the evolution of an array of innovative approaches, the application of which broadens the scope of experimentally feasible questions to include the interaction of proteins with diverse binding partners. The available array of modified and alternative approaches facilitates the analysis of complex cellular machinery and signaling networks that rely on multiple protein interactions. Such advances have facilitated the functional analysis of proteins on the genome level, a feat considered untenable a decade ago.
Notes20314472 0378-1119 Journal Article Review Review, Tutorial