This is an archive of papers published by the staff and faculty of Fox Chase Cancer Center. For questions about content, please contact Talbot Research Library
Last updated on
Base excision repair in mammalian cells
Methods in Molecular Biology (Totowa, NJ, United States). 2006 ;314(DNA Repair Protocols (2nd Edition)) :365-375
PMID: AN 2005:1186502
AbstractA rapid, convenient and safe in vitro assay system for base excision repair is described. Whole cell exts. are prepd. by detergent-based cell lysis and provide a vigorous activity of AP site repair. A circular DNA substrate is used for detection of both DNA polymerase b-dependent and proliferating cell nuclear antigen (PCNA)-dependent pathways. Repaired and unrepaired DNA substrates are sepd. by agarose gel electrophoresis as a linear DNA mol. and a nicked circular mol., resp., and detected by staining with SYBR Green I. This assay system does not require radioactive substrates or nucleotides, and provides a sensitivity in which 10 ng of a DNA substrate per reaction is sufficient for quant. repair anal. [on SciFinder (R)]
Notes3 Biochemical Genetics Department of Pharmacology,Fox Chase Cancer Center,Philadelphia,PA,USA. Journal 1064-3745 written in English.