This is an archive of papers published by the staff and faculty of Fox Chase Cancer Center. For questions about content, please contact Talbot Research Library
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Cell migration analyses within fibroblast-derived 3-D matrices
Methods in Molecular Biology. 2005 ;294 :79-93
AbstractResearch in cell biology often is based in tissue culturing cells on artificial substrates, such as plastic or glass. These artificial conditions are prone to distorting findings by persuading cells to adjust to artificial flat rigid surfaces. In contrast, the natural substrate for most cells in living organisms is the extracellular matrix (ECM), which is three-dimensional, complex, and dynamic in its molecular composition, and variable in pliability. Here we show how to measure the rates and directionality of fibroblasts motility within physiologically relevant in vivo-like cell-derived three-dimensional matrices.
NotesUnited States Journal Article