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Greger JG , Katz RA , Taganov K , Rall GF , Skalka AM
Transduction of terminally differentiated neurons by avian sarcoma virus
Journal of Virology. 2004 May;78(9) :4902-4906
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Recent studies have demonstrated that avian sarcoma virus (ASV) can transduce cycle-arrested cells. Here, we have assessed quantitatively the transduction efficiency of an ASV vector in naturally arrested mouse hippocampal neurons. This efficiency was determined by comparing the number of transduced cells after infection of differentiated neurons versus dividing progenitor cells. The results indicate that ASV is able to transduce these differentiated neurons efficiently and that this activity is not the result of infection of residual dividing cells. The transduction efficiency of the ASV vector was found to be intermediate between the relatively high and low efficiencies obtained with human immunodeficiency virus type I and murine leukemia virus vectors, respectively.
English Article KeyWords Plus: HUMAN-IMMUNODEFICIENCY-VIRUS; HEMATOPOIETIC STEM-CELLS; MURINE LEUKEMIA-VIRUS; LENTIVIRAL VECTOR; GENE-TRANSFER; NONDIVIDING CELLS; HIV-1 INTEGRATION; TYPE-1; EXPRESSION; INFECTION Katz, RA; *Fox* *Chase* Canc Ctr, Inst Canc Res, 333 Cottman Ave, Philadelphia, PA 19111 USA. Research Addresses: *Fox* *Chase* Canc Ctr, Inst Canc Res, Philadelphia, PA 19111 USA. Univ Penn, Sch Med, Grad Grp Cell & Mol Biol, Philadelphia, PA 19104 USA. Russian State Med Univ, Dept Mol Biol & Biotechnol, Moscow 117437, Russia. Cited References: BARSOV EV, 1996, J VIROL, V70, P3922 BUKRINSKY MI, 1992, P NATL ACAD SCI USA, V89, P6580 CHEN WY, 2000, STEM CELLS, V18, P352 DANIEL R, 2003, P NATL ACAD SCI USA, V100, P4778 DEGLON N, 2002, CURR TOP MICROBIOL, V261, P191 GEROLAMI R, 2000, CANCER GENE THER, V7, P1286 0022-538X