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Edwards Angela V , Davis Dorene L , Juraszek Amy L , Wessels Andy , Burch John BE
Transcriptional regulation in the mouse atrioventricular conduction system
Novartis Foundation Symposium. 2003 ;250(Development of the Cardiac Conduction System) :177-193
PMID: AN 2003:993906   
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We identified a GATA6 gene enhancer that selectively marks the developing atrioventricular conduction system (AVCS) in transgenic mice. This enhancer reads anterior/posterior and medial/lateral positional information early in the cardiogenic program and remains active in progressively more restricted subsets of heart cells leading up to AVCS formation. Addnl. expts. will be required to det. if the potential to be recruited into the AVCS is similarly restricted to a subset of myocardial cells early in the cardiogenic program or if this enhancer can also be activated de novo in cells that initially reside outside this field. We are using several strategies to identify factors that regulate this and other AVCS enhancers and hence govern AVCS function. We are also using this enhancer to make transgenic mice that express Cre, or an inducible form of Cre, to track lineages and to delete floxed genes in the developing or mature AVCS. This Cre/lox approach provides a means to deconstruct complex congenital heart phenotypes that involve the conduction system and to test whether genes are required to form the AVCS or to maintain AVCS function. Lastly, we are exploring strategies to isolate and analyze AVCS cells from normal and affected hearts. [on SciFinder (R)]
CAN 140:194280 3-4 Biochemical Genetics Department of Cell and Developmental Biology,Fox Chase Cancer Center,Philadelphia,PA,USA. Journal 1528-2511 written in English.