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Katz RA , Greger JG , Boimel P , Skalka AM
Human immunodeficiency virus type 1 DNA nuclear import and integration are mitosis independent in cycling cells
Journal of Virology. 2003 Dec;77(24) :13412-13417
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An essential step in human immunodeficiency virus type 1 (HIV-1) replication is the movement of the viral preintegration complex from the cytoplasm into the nucleus. The pathway(s) and timing for HIV-1 DNA nuclear entry in cycling cells have not been established. Here, we show that if cycling cells are infected before S phase, viral DNA can be integrated prior to passage of the host DNA replication fork through the integration site, as indicated by stable inheritance in both daughter cells. We conclude that efficient nuclear entry can occur independently of mitotic nuclear disassembly in cycling cells.
English Article KeyWords Plus: NONDIVIDING CELLS; PREINTEGRATION COMPLEXES; LENTIVIRAL VECTOR; SARCOMA VIRUS; IN-VIVO; HIV DNA; LOCALIZATION; TRANSDUCTION; INFECTION; FLAP Katz, RA; 333 Cottman Ave, Philadelphia, PA 19111 USA. Research Addresses: Univ Penn, Sch Med, Inst Canc Res, *Fox* *Chase* Canc Ctr, Philadelphia, PA 19104 USA. Univ Penn, Sch Med, Grad Grp Cell & Mol Biol, Philadelphia, PA 19104 USA. Cited References: BOUYACBERTOIA M, 2001, MOL CELL, V7, P1025 BUKRINSKY MI, 1993, NATURE, V365, P666 BUTLER SL, 2001, NAT MED, V7, P631 CHEN WY, 2000, STEM CELLS, V18, P352 COFFIN JM, 1997, RETROVIRUSES CULLEN BR, 2001, CELL, V105, P697 DVORIN JD, 2002, J VIROL, V76, P12087 FLINT SJ, 2000, PRINCIPLES VIROLOGY FOUCHIER RAM, 1999, ADV VIRUS RES, V52, P275 HAJIHOSSEINI M, 1993, EMBO J, V12, P4969 HATZIIOANNOU T, 2001, J VIROL, V75, P9526 KATZ RA, 2002, J VIROL, V76, P5422 LEWIS PF, 1994, J VIROL, V68, P510 LIMON A, 2002, J VIROL, V76, P10598 LIMON A, 2002, J VIROL, V76, P12078 NALDINI L, 1996, SCIENCE, V272, P263 ROE TY, 1993, EMBO J, V12, P2099 VANDEGRAAFF N, 2001, J VIROL, V75, P11253 ZENNOU V, 2000, CELL, V101, P173 Number of cited references: 19 Number of times cited: 0 Publisher: AMER SOC MICROBIOLOGY; 1752 N ST NW, WASHINGTON, DC 20036-2904 USA. 0022-538X