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Yang DH , Moss EG
Temporally regulated expression of Lin-28 in diverse tissues
Gene Expression Patterns. 2003 Dec;3(6) :719-726
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The gene lin-28 was originally identified through a mutant of the nematode Caenorhabditis elegans displaying defects in developmental timing. It is expressed stage-specifically in tissues throughout the animal and is required for cell fates to be expressed at the appropriate stage of larval development. lin-28 encodes a cytoplasmic protein with a unique pairing of RNA-binding motifs. Diverse animals possess Lin-28 homologues and mouse Lin-28 is expressed in embryos, embryonic stem cells and embryonal carcinoma cells, but not in some differentiated cell types. To assess whether mammalian Lin-28 may function as a developmental timing regulator, we examined adult and embryonic tissues of the mouse for its expression. We observed Lin-28 protein in many diverse tissues of the embryo through the period of organogenesis and that it persists in some tissues in the adult. In addition to an overall down-regulation during embryogenesis, in at least two tissues Lin-28 expression shows t! emporal regulation, as opposed to cell type or tissue-specific regulation: in the developing bronchial epithelium, where it is present in the developing lung and absent in the adult, and in a subset of cells developing along the crypt-villus axis of the intestine. Interestingly, unlike epithelia, cardiac and skeletal muscle continuously express Lin-28, suggesting an ongoing need for its activity there. We also observed that Lin-28 expression is repressed during the retinoic acid-induced differentiation of mouse P19 cells into neuronal cells, suggesting that down-regulation of Lin-28 in some tissues may occur in response to hormonal signals that govern development. (C) 2003 Elsevier B.V. All rights reserved.
English Article Moss, EG; Univ Med & Dent New Jersey, Dept Mol Biol, Stratford, NJ 08084 USA. Research Addresses: *Fox* *Chase* Canc Ctr, Philadelphia, PA 19111 USA. Cited References: AMBROS V, 2000, CURR OPIN GENET DEV, V10, P428 HUGHES SM, 2001, CURR BIOL, V11, PR237 MOSS EG, 2003, DEV BIOL, V258, P432 MOSS EG, 1997, CELL, V88, P637 SEGGERSON K, 2002, DEV BIOL, V243, P215 YANG DH, 2002, DEV BIOL, V251, P27 Number of cited references: 6 Number of times cited: 0 Publisher: ELSEVIER SCIENCE BV; PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS. 1567-133X