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Engelholm LH , List K , Netzel-Arnett S , Cukierman E , Mitola DJ , Aaronson H , Kjoller L , Larsen JK , Yamada KM , Strickland DK , Holmbeck K , Dano K , Birkedal-Hansen H , Behrendt N , Bugge TH
uPARAP/Endo180 is essential for cellular uptake of collagen and promotes fibroblast collagen adhesion
J Cell Biol. 2003 Mar 31;160(7) :1009-15
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Abstract
The uptake and lysosomal degradation of collagen by fibroblasts constitute a major pathway in the turnover of connective tissue. However, the molecular mechanisms governing this pathway are poorly understood. Here, we show that the urokinase plasminogen activator receptor-associated protein (uPARAP)/Endo180, a novel mesenchymally expressed member of the macrophage mannose receptor family of endocytic receptors, is a key player in this process. Fibroblasts from mice with a targeted deletion in the uPARAP/Endo180 gene displayed a near to complete abrogation of collagen endocytosis. Furthermore, these cells had diminished initial adhesion to a range of different collagens, as well as impaired migration on fibrillar collagen. These studies identify a central function of uPARAP/Endo180 in cellular collagen interactions.
Notes
22554950 0021-9525 Journal Article