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Dumont Patrick , Della Pietra Anthony , Murphy Maureen E
Methods to study p53-repressed promoters
Methods in Molecular Biology (Totowa, NJ, United States). 2003 ;234(p53 Protocols) :111-120
PMID: AN 2003:566301
AbstractThere is substantial evidence in the literature that, in addn. to functioning as an activator of transcription, the p53 tumor suppressor protein can also function as a sequence-specific transcriptional repressor of a sep. set of genes. However, elucidation of the mechanism whereby p53 functions as a transcriptional repressor has been obscured by the use of artificial assays to measure this activity; these assays include transient transfection analyses, where both p53 and target promoters are overexpressed. This chapter describes alternative approaches for the definition of sequence elements that mediate transcriptional repression by p53. These include the McKay (immunobinding) assay, which measures the in vitro binding of large fragments of DNA, as well as chromatin immunopptns. (ChIPs), which measure in vivo binding. The use of such assays should better define the mechanism of transcriptional repression by p53 and should aid in the elucidation of the contribution of this activity to p53-dependent growth arrest and programmed cell death (apoptosis). [on SciFinder (R)]
NotesCAN 139:225082 3-1 Biochemical Genetics Department of Pharmacology,Fox Chase Cancer Center,Philadelphia,PA,USA. Journal 1064-3745 written in English.