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Pachuk CJ , Ciccarelli RB , Samuel M , Bayer ME , Troutman RD , Zurawski DV , Schauer JI , Higgins TJ , Weiner DB , Sosnoski DM , Zurawski VR , Satishchandran C
Characterization of a new class of DNA delivery complexes formed by the local anesthetic bupivacaine
Biochimica Et Biophysica Acta-Biomembranes. 2000 Sep 29;1468(1-2) :20-30
AbstractBupivacaine, a local anesthetic and cationic amphiphile, forms stable liposomal-like structures upon direct mixing with plasmid DNA in aqueous solutions. These structures are on the order of 50 70 nm as determined by scanning electron microscopy, and are homogeneous populations as analyzed by density gradient centrifugation. The DNA within these structures is protected from nuclease degradation and UV- induced damage in vitro. Bupivacaine:DNA complexes have a negative zeta potential (surface charge), homogeneous nature, and an ability to rapidly assemble in aqueous solutions. Bupivacaine:DNA complexes, as well as similar complexes of DNA with other local anesthetics, have the potential to be a novel class of DNA delivery agents for gene therapy and DNA vaccines. (C) 2000 Elsevier Science B.V. All rights reserved.
NotesTimes Cited: 3 English Article 363CF BBA-BIOMEMBRANES