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Chumley MJ , Dal Porto JM , Kawaguchi S , Cambier JC , Nemazee D , Hardy RR
A V(H)11V(kappa)9B cell antigen receptor drives generation of CD5(+) B cells both in vivo and in vitro
Journal of Immunology. 2000 May 1;164(9) :4586-4593
AbstractB lymphocytes can be divided into different subpopulations, some with distinctive activation requirements and probably mediating specialized functions, based on surface phenotype and/or anatomical location, but the origins of most of these populations remain poorly understood. B cells constrained by transgenesis to produce an Ag receptor derived from a conventional (B-2) type cell. develop a B-2 phenotype, whereas cells from mice carrying a B-1-derived receptor acquire the B-1 phenotype. In this study transgenic enforced expression of a B cell receptor (mu/kappa) originally isolated from a CD5(+) (B- 1a) B cell generates B-1 phenotype cells in bone marrow cultures that show a distinctive B-l function, survival in culture. Despite their autoreactivity, we find no evidence for receptor editing or that the paucity of B-2 cells is the result of tolerance-induced selection. Finally, Ca2+ mobilization studies reveal a difference between transgenic B-1 cells in spleen and peritoneal cavity, with cells in spleen much more responsive to anti-B cell receptor cross-linking. We discuss these results in terms of specificity vs lineage models for generation of distinctive B cell subpopulations.
NotesTimes Cited: 18 English Article 306XQ J IMMUNOL