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McNamara R , Ariasmendoza F , Brown TR
Investigation of Broad Resonances in P-31 Nmr-Spectra of the Human Brain in-Vivo
Nmr in Biomedicine. 1994 Aug;7(5) :237-242
PMID: ISI:A1994PJ87100006   
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Broad resonances that lie underneath the familiar small molecule profile of in vivo P-31 NMR spectra can make accurate spectral integration of these mobile phosphates difficult. The two major broad components are the phosphate contained in the hydroxyapatite in cranial bone and the phosphodiester moiety in partially mobile membrane phospholipids. They can be removed with post-acquisition processing but this results in distortion of lineshapes and intensities and interferes with accurate quantitation. We have employed an off-resonance saturation procedure to eliminate the bone resonance and isolate the signal from the membrane phospholipids by subtraction. Selective saturation of the phospholipid resonance increases the clarity of the sharp peaks downfield from the phosphocreatine peak. Selective saturation 3-D chemical shift imaging techniques were used to create a localized phospholipid profile of the entire brain simultaneously. Monitoring localized phospholipid concentration may be important in studying demyelinating diseases.
English Article PJ871 NMR BIOMED