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Kariv I , Hardy RR , Hayakawa K
2 Distinct Non-T Helper Type-2 Interleukin-4(+) Cell Subsets in Mice as Revealed by Single-Cell Cytokine Analysis
European Journal of Immunology. 1994 Mar;24(3) :549-557
PMID: ISI:A1994NB71800008   
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We have previously defined four murine CD4(+) peripheral T cell subsets, fractions (Fr.) I - IV, based on expression of the 6C10 and 3G11 determinants (Hayakawa, K. and Hardy, R. R., J. Exp. Med. 1988. 168: 1825). These subsets also show distinctive levels of other cell surface markers: the two minor subsets, Fr. III and Fr. IV, are both CD45RB(low/-), L-selectin (Mel- 14)(-) and CD44(hi), characteristic of secondary T cells. The patterns and levels of cytokine production by individual cells in each subset were determined by bioassay for interleukin (IL)-2/IL-4 or IL-4/interferon (IFN)-gamma production after anti-CD3 stimulation. Our data revealed that these four phenotypically defined subsets largely coincide with clusters of cells showing uniform distinctive cytokine profiles, i.e. IL-2(+)/IFN-gamma(-)/IL-4(-) (Fr. I and Fr. II, L-selectin(+)), IL-2(+)/IFN-gamma(+)/IL-4(+) (Fr. III, L-selectin(-)), and IL- 2(-)/IFN-gamma(low/-)/IL-4(+) (Fr.IV, L-selectin(-)). Besides these subsets, an L-selectin-negative cell subfraction within Fr. II appears to represent a transitional population between the IL-2(+)/IFN-gamma(-)/IL-4(-) stage and the IL-2(+)/IFN- gamma(+)/IL-4(+) stage. Taken together, these results demonstrate the presence of two IL-4(+) secondary T cell subsets with distinct cytokine production patterns, and show that the majority of IL-4(+) cells found in healthy adult laboratory mice co-produce IFN-gamma, and thus are not typical T helper type 2 cells.
English Article NB718 EUR J IMMUNOL