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Dube DK , Dube S , Erensoy S , Jones B , Bryzgornia V , Spicer T , Love J , Saksena N , Lechat MF , Shrager DI , Dosik H , Glaser J , Levis W , Blattner W , Montagna R , Blumberg BS , Poiesz BJ
Serological and Nucleic-Acid Analyses for Hiv and Htlv Infection on Archival Human Plasma Samples from Zaire
Virology. 1994 Jul;202(1) :379-389
PMID: ISI:A1994NQ85800039   
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In order to better understand the genomic diversity and molecular phylogeny of the human retroviruses, the plasmas from 250 Zairean patients collected in 1969 were tested for antibodies to human T-cell lymphoma and human immunodeficiency viruses (HTLV or HIV) using ELISA and confirmatory Western blots and for viral nucleic acids by reverse transcriptase- directed PCR (RT-PCR). Interestingly, none of the patients was confirmed positive for HIV, even though this region is now endemic for HIV-1. However, 74 (30%) and 3 (1%) of the samples were positive for antibodies to HTLV-I and II, respectively. Forty-four of 74 (59%) Western blot-positive Zairean samples were RT-PCR positive for HTLV-I, while 1 of 3 (33%) of HTLV-II- seropositive samples was RT-PCR positive. On the contrary, none of the Western blot-negative or indeterminate samples were RT- PCR positive for either HTLV-I or HTLV-II, We have cloned and sequenced 140 bp of the pol gene flanked by SK110/SK111 from 8 HTLV-I- and 1 HTLV-II-positive archival samples from Zaire, The HTLV-I isolates from Zaire cluster together as a phylogenetic group, diverging from the prototype Japanese HTLV-I (ATK) by a range of 1.4 to 3.6%. Their close homology to some African STLV-I isolates suggests relatively recent interspecies transmission. The Zairean HTLV-II isolate is closely grouped with the HTLV-II substrain of isolates found in Paleo- Amerindians of the New World, making it unlikely that it represents an endemic African strain. (C) 1994 Academic Press, Inc
English Article NQ858 VIROLOGY