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Impact of Sun Exposure and Tanning Patterns on Next-Generation Sequencing Mutations in Melanoma
J Surg Res. 2020 Oct;254 :147-153
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BACKGROUND: For the past 30 y, the incidence rate of malignant melanoma has risen steadily. Ultraviolet radiation exposure has been identified as the most prevalent modifiable risk factor for melanoma. Here, next-generation sequencing was used to analyze the relationship between multiple sun exposure factors and select cancer-related genes to determine the relationship of sun exposure on the molecular profiles of melanomas. METHODS: The collection and analysis of study samples were approved by the institutional review board. The patient cohort consisted of 173 patients whose melanoma tissue samples underwent next-generation sequencing analysis for somatic mutations of 50 cancer-related genes. Univariate and multivariate analyses were conducted. RESULTS: Patients with a history of blistering sunburn had an absolute mutation incidence of 1.67 mutations per patient, compared with patients without a history of blistering sunburn, who had an absolute mutation incidence of 1.16 mutations per patient (P = 0.028). A BRAF mutation was found in more tumors of patients who reported visiting a tanning salon (57.14%), compared with those who had not (18.75%; P = 0.0463). Patients with a previous history of skin cancer were more likely to have a CDKN2A mutation (20.83%), compared with those without a previous history of skin cancer (7.76%; P = 0.0292). CONCLUSIONS: The trends seen in the molecular profiles of melanomas with respect to various sun exposure factors suggest that sun exposure impacts genetic makeup. Considering the increase in absolute mutation incidence in patients with a history of blistering sunburn suggests that additional genes may contribute to the pathology of malignancy. Future studies will use the unique molecular profiles of melanomas to personalize patient treatments.
1095-8673 Loo, Kimberly Soliman, Iman Renzetti, Madelyn Li, Tianyu Wu, Hong Reddy, Sanjay Olszanski, Anthony J Farma, Jeffrey M Journal Article United States Research Support, Non-U.S. Gov't J Surg Res. 2020 Oct;254:147-153. doi: 10.1016/j.jss.2020.04.021. Epub 2020 May 21.