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Muench DE , Olsson A , Ferchen K , Pham G , Serafin RA , Chutipongtanate S , Dwivedi P , Song BB , Hay S , Chetal K , Trump-Durbin LR , Mookerjee-Basu J , Zhang KJ , Yu JNC , Lutzko C , Myers KC , Nazor KL , Greis KD , Kappes DJ , Way SS , Salomonis N , Grimes HL
Mouse models of neutropenia reveal progenitor-stage-specific defects
Nature. 2020 Jun;582(7810) :109-114
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Abstract
Advances in genetics and sequencing have identified a plethora of disease-associated and disease-causing genetic alterations. To determine causality between genetics and disease, accurate models for molecular dissection are required; however, the rapid expansion of transcriptional populations identified through single-cell analyses presents a major challenge for accurate comparisons between mutant and wild-type cells. Here we generate mouse models of human severe congenital neutropenia (SCN) using patient-derived mutations in the GFI1 transcription factor. To determine the effects of SCN mutations, we generated single-cell references for granulopoietic genomic states with linked epitopes(1), aligned mutant cells to their wild-type equivalents and identified differentially expressed genes and epigenetic loci. We find that GFI1-target genes are altered sequentially, as cells go through successive states of differentiation. These insights facilitated the genetic rescue of granulocytic specification but not post-commitment defects in innate immune effector function, and underscore the importance of evaluating the effects of mutations and therapy within each relevant cell state. Mouse models of severe congenital neutropenia using patient-derived mutations in the GFI1 locus are used to determine the mechanisms by which the disease progresses.
Notes
Muench, David E. Olsson, Andre Ferchen, Kyle Pham, Giang Serafin, Rachel A. Chutipongtanate, Somchai Dwivedi, Pankaj Song, Baobao Hay, Stuart Chetal, Kashish Trump-Durbin, Lisa R. Mookerjee-Basu, Jayati Zhang, Kejian Yu, Jennifer C. Lutzko, Carolyn Myers, Kasiani C. Nazor, Kristopher L. Greis, Kenneth D. Kappes, Dietmar J. Way, Sing Sing Salomonis, Nathan Grimes, H. Leighton 1476-4687 Nature. 2020 Jun;582(7810):109-114. doi: 10.1038/s41586-020-2227-7. Epub 2020 Apr 22.