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Liu F , Dai S , Feng D , Peng X , Qin Z , Kearns AC , Huang W , Chen Y , Ergun S , Wang H , Rappaport J , Bryda EC , Chandrasekhar A , Aktas B , Hu H , Chang SL , Gao B , Qin X
Versatile cell ablation tools and their applications to study loss of cell functions
Cell Mol Life Sci. 2019 Jul 29;76(23) :4725-4743
PMID: 31359086    PMCID: PMC6858955    URL: https://www.ncbi.nlm.nih.gov/pubmed/31359086
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Targeted cell ablation is a powerful approach for studying the role of specific cell populations in a variety of organotypic functions, including cell differentiation, and organ generation and regeneration. Emerging tools for permanently or conditionally ablating targeted cell populations and transiently inhibiting neuronal activities exhibit a diversity of application and utility. Each tool has distinct features, and none can be universally applied to study different cell types in various tissue compartments. Although these tools have been developed for over 30 years, they require additional improvement. Currently, there is no consensus on how to select the tools to answer the specific scientific questions of interest. Selecting the appropriate cell ablation technique to study the function of a targeted cell population is less straightforward than selecting the method to study a gene's functions. In this review, we discuss the features of the various tools for targeted cell ablation and provide recommendations for optimal application of specific approaches.
1420-9071 Liu, Fengming Dai, Shen Feng, Dechun Peng, Xiao Qin, Zhongnan Kearns, Alison C Huang, Wenfei Chen, Yong Ergun, Suleyman Wang, Hong Rappaport, Jay Bryda, Elizabeth C Chandrasekhar, Anand Aktas, Bertal Hu, Hongzhen Chang, Sulie L Gao, Bin Qin, Xuebin R21od024931/od P40 od011062/od R21AA024984/National Institute on Alcohol Abuse and Alcoholism R21 DA043448/National Institute on Drug Abuse R01 HL130233/National Heart, Lung, and Blood Institute R01HL141132/National Heart, Lung, and Blood Institute Journal Article Review Switzerland Cell Mol Life Sci. 2019 Jul 29. pii: 10.1007/s00018-019-03243-w. doi: 10.1007/s00018-019-03243-w.