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Yadavalli SS , Xiao Q , Sherman SE , Hasley WD , Klein ML , Goulian M , Percec V
Bioactive cell-like hybrids from dendrimersomes with a human cell membrane and its components
Proc Natl Acad Sci U S A. 2019 Jan 15;116(3) :744-752
PMID: 30591566    PMCID: PMC6338876   
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Cell-like hybrids from natural and synthetic amphiphiles provide a platform to engineer functions of synthetic cells and protocells. Cell membranes and vesicles prepared from human cell membranes are relatively unstable in vitro and therefore are difficult to study. The thicknesses of biological membranes and vesicles self-assembled from amphiphilic Janus dendrimers, known as dendrimersomes, are comparable. This feature facilitated the coassembly of functional cell-like hybrid vesicles from giant dendrimersomes and bacterial membrane vesicles generated from the very stable bacterial Escherichia coli cell after enzymatic degradation of its outer membrane. Human cells are fragile and require only mild centrifugation to be dismantled and subsequently reconstituted into vesicles. Here we report the coassembly of human membrane vesicles with dendrimersomes. The resulting giant hybrid vesicles containing human cell membranes, their components, and Janus dendrimers are stable for at least 1 y. To demonstrate the utility of cell-like hybrid vesicles, hybrids from dendrimersomes and bacterial membrane vesicles containing YadA, a bacterial adhesin protein, were prepared. The latter cell-like hybrids were recognized by human cells, allowing for adhesion and entry of the hybrid bacterial vesicles into human cells in vitro.
1091-6490 Yadavalli, Srujana S Orcid: 0000-0002-4335-1973 Xiao, Qi Orcid: 0000-0002-6470-0407 Sherman, Samuel E Hasley, William D Klein, Michael L Goulian, Mark Percec, Virgil R01 GM080279/GM/NIGMS NIH HHS/United States Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. United States Proc Natl Acad Sci U S A. 2019 Jan 15;116(3):744-752. doi: 10.1073/pnas.1811307116. Epub 2018 Dec 27.