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Gangolli RA , Devlin SM , Gerstenhaber JA , Lelkes PI , Yang M
A Bilayered Poly (Lactic-Co-Glycolic Acid) Scaffold Provides Differential Cues for the Differentiation of Dental Pulp Stem Cells
Tissue Eng Part A. 2019 Feb;25(3-4) :224-233
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<b>Introduction:</b> Regenerative Endodontics (RE) is a clinical procedure that aims to regenerate the dentin-pulp complex (DPC) inside the root canal. Current clinical outcomes of RE are unpredictable, and the regenerated tissue lacks the spatial organization seen in normal DPC. The purpose of this study was to develop and characterize in vitro a bilayered scaffold with distinct porosities on each side that supports differential penetration and odontoblastic differentiation of cultured human dental pulp stem cells (DPSCs). (DPSCs). <b>Materials and Methods:</b> Bilayered scaffolds were manufactured from poly (lactic-co-glycolic acid) (PLGA) using diffusion induced phase separation (DIPS). The layers were generated separately from 12% and 20% (w/v) PLGA and combined by lamination. Scaffold morphology was assessed by scanning electron microscopy. Human DPSCs were cultured on either side of the scaffold. Cell proliferation, viability, and penetration into the scaffolds were analyzed biochemically and by confocal imaging. Odontoblastic differentiation of the DPCSs and mineralization were analyzed by qRT-PCR, quantification of Alizarin red staining, energy dispersive X-ray spectroscopy (EDX), and Fourier transform infrared spectroscopy (FTIR). <b>Results:</b> The bilayered scaffold (thicknesses 240 +/- 10 mum) contained continuous channels with gradual taper. Channel diameters ranged from 45 microm to 10 microm on the open side (20% PLGA side), and 10 microm to 5 microm on the closed side (12% PLGA side). While proliferating equally on either scaffold surfaces, DPSCs penetrated into the open side and through the entire thickness of that layer in 14 days. By contrast, the closed side limited cell penetration into the scaffold but significantly promoted odontogenic differentiation in the absence of odontogenic induction medium. <b>Conclusions:</b> Bilayered scaffolds provide spatial control of differential DPSC penetration and odontogenic differentiation, thus providing a potential scaffold for DPC regeneration.
1937-335x Gangolli, Riddhi A Devlin, Sean M Gerstenhaber, Jonathan Arye Lelkes, Peter Istvan Yang, Maobin Journal Article United States Tissue Eng Part A. 2019 Feb;25(3-4):224-233. doi: 10.1089/ten.TEA.2018.0041. Epub 2018 Sep 13.