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Kasimova MA , Yazici A , Yudin Y , Granata D , Klein ML , Rohacs T , Carnevale V
Ion Channel Sensing: Are Fluctuations the Crux of the Matter?
J Phys Chem Lett. 2018 Mar 15;9(6) :1260-1264
PMID: 29439562    PMCID: PMC6310152    URL: https://www.ncbi.nlm.nih.gov/pubmed/29439562
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The nonselective cation channel TRPV1 is responsible for transducing noxious stimuli into action potentials propagating through peripheral nerves. It is activated by temperatures greater than 43 degrees C, while remaining completely nonconductive at temperatures lower than this threshold. The origin of this sharp response, which makes TRPV1 a biological temperature sensor, is not understood. Here we used molecular dynamics simulations and free energy calculations to characterize the molecular determinants of the transition between nonconductive and conductive states. We found that hydration of the pore and thus ion permeation depends critically on the polar character of its molecular surface: in this narrow hydrophobic enclosure, the motion of a polar side-chain is sufficient to stabilize either the dry or wet state. The conformation of this side-chain is in turn coupled to the hydration state of four peripheral cavities, which undergo a dewetting transition at the activation temperature.
1948-7185 Kasimova, Marina A Yazici, Aysenur Yudin, Yevgen Granata, Daniele Klein, Michael L Rohacs, Tibor Carnevale, Vincenzo ORCID: http://orcid.org/0000-0002-1918-8280 Journal Article United States J Phys Chem Lett. 2018 Feb 27:1260-1264. doi: 10.1021/acs.jpclett.7b03396.