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Xu Y , Xia J , Liu S , Stein S , Ramon C , Xi H , Wang L , Xiong X , Zhang L , He D , Yang W , Zhao X , Cheng X , Yang X , Wang H
Endocytosis and membrane receptor internalization: implication of F-BAR protein Carom
Front Biosci (Landmark Ed). 2017 Mar 1;22 :1439-1457
PMID: 28199211    PMCID: PMC5315272   
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Abstract
Endocytosis is a cellular process mostly responsible for membrane receptor internalization. Cell membrane receptors bind to their ligands and form a complex which can be internalized. We previously proposed that F-BAR protein initiates membrane curvature and mediates endocytosis via its binding partners. However, F-BAR protein partners involved in membrane receptor endocytosis and the regulatory mechanism remain unknown. In this study, we established database mining strategies to explore mechanisms underlying receptor-related endocytosis. We identified 34 endocytic membrane receptors and 10 regulating proteins in clathrin-dependent endocytosis (CDE), a major process of membrane receptor internalization. We found that F-BAR protein FCHSD2 (Carom) may facilitate endocytosis via 9 endocytic partners. Carom is highly expressed, along with highly expressed endocytic membrane receptors and partners, in endothelial cells and macrophages. We established 3 models of Carom-receptor complexes and their intracellular trafficking based on protein interaction and subcellular localization. We conclude that Carom may mediate receptor endocytosis and transport endocytic receptors to the cytoplasm for receptor signaling and lysosome/proteasome degradation, or to the nucleus for RNA processing, gene transcription and DNA repair.
Notes
1093-4715 Xu, Yanjie Xia, Jixiang Liu, Suxuan Stein, Sam Ramon, Cueto Xi, Hang Wang, Luqiao Xiong, Xinyu Zhang, Lixiao He, Dingwen Yang, William Zhao, Xianxian Cheng, Xiaoshu Yang, Xiaofeng Wang, Hong R01 HL108910/HL/NHLBI NIH HHS/United States R01 HL077288/HL/NHLBI NIH HHS/United States R01 HL117654/HL/NHLBI NIH HHS/United States R01 HL131460/HL/NHLBI NIH HHS/United States R01 HL082774/HL/NHLBI NIH HHS/United States R01 HL110764/HL/NHLBI NIH HHS/United States R01 HL132399/HL/NHLBI NIH HHS/United States R01 HL094451/HL/NHLBI NIH HHS/United States F32 HL009445/HL/NHLBI NIH HHS/United States R01 HL067033/HL/NHLBI NIH HHS/United States R01 HL116917/HL/NHLBI NIH HHS/United States Journal Article Review United States Front Biosci (Landmark Ed). 2017 Mar 1;22:1439-1457.