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Sitarek P , Skala E , Toma M , Wielanek M , Szemraj J , Skorski T , Bialas AJ , Sakowicz T , Kowalczyk T , Radek M , Wysokinska H , Sliwinski T
Transformed Root Extract of Leonurus sibiricus Induces Apoptosis through Intrinsic and Extrinsic Pathways in Various Grades of Human Glioma Cells
Pathol Oncol Res. 2017 Jul;23(3) :679-687
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Abstract
This study determines the influence of transformed root (TR) extract of Leonurus sibiricus L. on various grades (I-III) of human glioma cells derived from patients. This plant occurs in southern Asia and Siberia and is widely used as a medicinal plant with various biological activities. Chromatographic profile of TR extract have revealed the presence of various polyphenolic compounds (4-hydroxybenzoic acid, gentisic acid, vanilic acid, 1,3-dicaffeoylquinic acid, alpha-resorcylic acid). We found TR root extract to have antiproliferative activity on glioma cells after 24 h of treatment. TR root extract induces apoptosis on various grades (I-III) of human glioma cells by the generation of reactive oxygen species (ROS) along with concurrent loss of mitochondrial membrane potential, enhanced S and G2/M phases of the cell cycle, and altered mRNA levels of Bax, Bcl-2, p53, Cas-3, Cas-8 and Cas-9 factors involved in apoptosis. This work for the first time demonstrate that TR extract from L. sibiricus root has the potential to activate apoptosis in grade I-III human glioma cells through the intrinsic and extrinsic pathways.
Notes
1532-2807 Sitarek, Przemyslaw Skala, Ewa Toma, Monika Wielanek, Marzena Szemraj, Janusz Skorski, Tomasz Bialas, Adam J Sakowicz, Tomasz Kowalczyk, Tomasz Radek, Maciej Wysokinska, Halina Sliwinski, Tomasz Journal Article Netherlands Pathol Oncol Res. 2017 Jul;23(3):679-687. doi: 10.1007/s12253-016-0170-6. Epub 2016 Dec 28.