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Lazarovici P , Lahiani A , Gincberg G , Haham D , Marcinkiewicz C , Lelkes PI
Nerve Growth Factor-Induced Angiogenesis: 2. The Quail Chorioallantoic Membrane Assay
Methods Mol Biol. 2018 Jan;1727 :251-259
PMID: 29222787 URL: https://www.ncbi.nlm.nih.gov/pubmed/29222787
AbstractThe avian chorioallantoic membrane (CAM) is a simple, highly vascularized extraembryonic membrane, which performs multiple functions during embryonic development. Therefore, the models of chicken and quail assays represent robust experimental platforms to study angiogenesis, which reflects perturbation of the entire vascular tree. This experimental approach, when combined with fractal morphometry, is sensitive to changes in vascular branching pattern and density. Nerve growth factor is a neurotrophin promoting angiogenesis in CAM models. Here, we provide a detailed protocol of the quail CAM, shell-less model, to study nerve growth factor effects on blood capillary sprouting. The quail CAM assay may be beneficial in investigations of cellular and molecular aspects of neurotrophin-induced angiogenesis and for developing novel anti-angiogenesis and anticancer therapies.
Notes1940-6029 Lazarovici, Philip Lahiani, Adi Gincberg, Galit Haham, Dikla Marcinkiewicz, Cezary Lelkes, Peter I Journal Article United States Methods Mol Biol. 2018;1727:251-259. doi: 10.1007/978-1-4939-7571-6_19.