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Castaneda CA , Wolfson NA , Leng KR , Kuo YM , Andrews AJ , Fierke CA
Histone deacetylase 8 substrate selectivity is determined by long- and short-range interactions leading to enhanced reactivity for full-length histone substrates compared to peptides
J Biol Chem. 2017 Dec 29;292(52) :21568-21577
PMID: 29109148    PMCID: PMC5766737    URL: https://www.ncbi.nlm.nih.gov/pubmed/29109148
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Histone deacetylases (HDACs) catalyze deacetylation of acetyl-lysine residues within proteins. To date, HDAC substrate specificity and selectivity have been largely estimated using peptide substrates. However, it is unclear whether peptide substrates accurately reflect the substrate selectivity of HDAC8 toward full-length proteins. Here, we compare HDAC8 substrate selectivity in the context of peptides, full-length proteins, and protein-nucleic acid complexes. We demonstrate that HDAC8 catalyzes deacetylation of tetrameric histone (H3/H4) substrates with catalytic efficiencies that are 40- to 300-fold higher than those for corresponding peptide substrates. Thus, we conclude that additional contacts with protein substrates enhance catalytic efficiency. However, the catalytic efficiency decreases for larger multi-protein complexes. These differences in HDAC8 substrate selectivity for peptides and full-length proteins suggest that HDAC8 substrate preference is based on a combination of short- and long-range interactions. In summary, this work presents detailed kinetics for HDAC8-catalyzed deacetylation of singly-acetylated, full-length protein substrates, revealing that HDAC8 substrate selectivity is determined by multiple factors. These insights provide a foundation for understanding recognition of full-length proteins by HDACs.
1083-351x Castaneda, Carol Ann ORCID: http://orcid.org/0000-0002-1233-4062 Wolfson, Noah A Leng, Katherine R Kuo, Yin-Ming Andrews, Andrew J Fierke, Carol A ORCID: http://orcid.org/0000-0002-1481-0579 T32 GM008353/GM/NIGMS NIH HHS/United States T32 GM008597/GM/NIGMS NIH HHS/United States Journal Article United States J Biol Chem. 2017 Nov 6. pii: M117.811026. doi: 10.1074/jbc.M117.811026.