FCCC LOGO Faculty Publications
Negendank W , Li CW , Shaller KP , MurphyBoesch J , Brown TR
Phospholipid metabolites in H-1-decoupled P-31 MRS in vivo in human cancer: Implications for experimental models and clinical studies
Anticancer Research. 1996 May-Jun;16(3B) :1539-1544
PMID: ISI:A1996UV17100028   
Back to previous list
Abstract
The use of P-31 MRS in clinical cancer research has been hampered by both poor anatomic localization of spectra and poor resolution of overlapping signals. We found that accurate localization using 3D chemical shift imaging and improved resolution using H-1-decoupling and nuclear Overhauser- enhancement (NOE) increased signal-to-noise and permitted resolution of separate components within phosphomonoester (PME) and phosphodiester (PDE) regions. Fifty-three cancers of different types (lymphoma, sarcoma, adenocarcinoma) had the following common features: (1) phosphoethanolamine the dominant PME; (2) glycerophospho-ethanolamine and -choline rarely detected; (3) a broad PDE signal probably from membrane phospholipids; and (4) prominent nucleoside triphosphates. H-1- decoupling with NOE-enchancement permitted us to obtain new information about in vivo metabolism in human cancers; generate new hypotheses and help guide development of experimental models appropriate to test them; and provider a firm basis with which to examine clinical uses of P-31 MRS.
Notes
Times Cited: 8 English Article UV171 ANTICANCER RES