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Xiao Q , Sherman SE , Wilner SE , Zhou X , Dazen C , Baumgart T , Reed EH , Hammer DA , Shinoda W , Klein ML , Percec V
Janus dendrimersomes coassembled from fluorinated, hydrogenated, and hybrid Janus dendrimers as models for cell fusion and fission
Proc Natl Acad Sci U S A. 2017 Aug 22;114(34) :E7045-e7053
PMID: 28784782    PMCID: PMC5576825    URL: https://www.ncbi.nlm.nih.gov/pubmed/28784782
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Abstract
A three-component system of Janus dendrimers (JDs) including hydrogenated, fluorinated, and hybrid hydrogenated-fluorinated JDs are reported to coassemble by film hydration at specific ratios into an unprecedented class of supramolecular Janus particles (JPs) denoted Janus dendrimersomes (JDSs). They consist of a dumbbell-shaped structure composed of an onion-like hydrogenated vesicle and an onion-like fluorinated vesicle tethered together. The synthesis of dye-tagged analogs of each JD component enabled characterization of JDS architectures with confocal fluorescence microscopy. Additionally, a simple injection method was used to prepare submicron JDSs, which were imaged with cryogenic transmission electron microscopy (cryo-TEM). As reported previously, different ratios of the same three-component system yielded a variety of structures including homogenous onion-like vesicles, core-shell structures, and completely self-sorted hydrogenated and fluorinated vesicles. Taken together with the JDSs reported herein, a self-sorting pathway is revealed as a function of the relative concentration of the hybrid JD, which may serve to stabilize the interface between hydrogenated and fluorinated bilayers. The fission-like pathway suggests the possibility of fusion and fission processes in biological systems that do not require the assistance of proteins but instead may result from alterations in the ratios of membrane composition.
Notes
1091-6490 Xiao, Qi ORCID: http://orcid.org/0000-0002-6470-0407 Sherman, Samuel E Wilner, Samantha E Zhou, Xuhao Dazen, Cody Baumgart, Tobias Reed, Ellen H Hammer, Daniel A Shinoda, Wataru Klein, Michael L Percec, Virgil Journal Article United States Proc Natl Acad Sci U S A. 2017 Aug 22;114(34):E7045-E7053. doi: 10.1073/pnas.1708380114. Epub 2017 Aug 7.