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Jennings MC , Forman ME , Duggan SM , Minbiole KPC , Wuest WM
Efflux Pumps May Not Be the Major Drivers of QAC Resistance in Methicillin-Resistant Staphylococcus aureus
Chembiochem. 2017 Aug 17;18(16) :1573-1577
PMID: 28510317    PMCID: PMC5664203    URL: https://www.ncbi.nlm.nih.gov/pubmed/28510317
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Quaternary ammonium compounds (QACs) are commonly used antiseptics that are now known to be subject to bacterial resistance. The prevalence and mechanisms of such resistance, however, remain underexplored. We investigated a variety of QACs, including those with multicationic structures (multiQACs), and the resistance displayed by a variety of Staphylococcus aureus strains with and without genes encoding efflux pumps, the purported main driver of bacterial resistance in MRSA. Through MIC, kinetic, and efflux-based assays, we find that neither the qacR/qacA system present in S. aureus nor another efflux pump system is the main reason for bacterial resistance to QACs. Our findings suggest that membrane composition is the predominant driver that allows CA-MRSA to withstand the assault of conventional QAC antiseptics.
1439-7633 Jennings, Megan C Forman, Megan E Duggan, Stephanie M Minbiole, Kevin P C Wuest, William M Journal Article Germany Chembiochem. 2017 May 16. doi: 10.1002/cbic.201700233.