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Wang X , Li M , Gao Y , Gao J , Yang W , Liang H , Ji Q , Li Y , Liu H , Huang J , Cheng T , Yuan W
Rheb1-mTORC1 maintains macrophage differentiation and phagocytosis in mice
Exp Cell Res. 2016 Jun 10;344(2) :219-28
PMID: 27163399 URL: https://www.ncbi.nlm.nih.gov/pubmed/27163399
AbstractRas homolog enriched in brain (Rheb1) is a small GTPase and is known to be a direct activator of mTORC1. Dysregulation of Rheb1 has been shown to impair the cellular-energetic state and cell homeostasis. However, the role of Rheb1 in monocytes/macrophages differentiation and maturation is not clear. Here, we investigate the role of Rheb1 in mouse myelopoiesis using a Rheb1 conditional deletion murine model. We found that the absolute number of macrophages decreased in the bone marrow (BM) of Rheb1-deficient mice. Loss of Rheb1 inhibited the monocyte-to-macrophage differentiation process. Additionally, Rheb1 deletion reduced phagocytosis ability of macrophages by inhibiting the mTORC1 signaling pathway. Furthermore, 3BDO (an activator of mTORC1) rescued the phagocytosis ability of Rheb1-deficient macrophages. Thus, Rheb1 is critical for macrophage production and phagocytosis and executes these activities possibly via mTORC1-dependent pathway.
NotesWang, Xiaomin Li, Minghao Gao, Yanan Gao, Juan Yang, Wanzhu Liang, Haoyue Ji, Qing Li, Yanxin Liu, Hanzhi Huang, Jian Cheng, Tao Yuan, Weiping United States Exp Cell Res. 2016 Jun 10;344(2):219-28. doi: 10.1016/j.yexcr.2016.04.017. Epub 2016 May 7.