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Preparation of Extracellular Matrices Produced by Cultured and Primary Fibroblasts
Curr Protoc Cell Biol. 2016 Jun 01;71 :10 9 1-10 9 34
PMID: 27245425    PMCID: PMC5058441    URL: https://www.ncbi.nlm.nih.gov/pubmed/27245425
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Fibroblasts secrete and organize extracellular matrix (ECM), which provides structural support for their adhesion, migration, and tissue organization, besides regulating cellular functions such as growth and survival. Cell-to-matrix interactions are vital for vertebrate development. Disorders in these processes have been associated with fibrosis, developmental malformations, cancer, and other diseases. This unit describes a method for preparing a three-dimensional matrix derived from fibroblastic cells; the matrix is three-dimensional, cell and debris free, and attached to a two-dimensional culture surface. Cell adhesion and spreading are normal on these matrices. This matrix can also be compressed into a two-dimensional matrix and solubilized to study the matrix biochemically. (c) 2016 by John Wiley & Sons, Inc.
Franco-Barraza, Janusz Beacham, Dorothy A Amatangelo, Michael D Cukierman, Edna United States Curr Protoc Cell Biol. 2016 Jun 1;71:10.9.1-10.9.34. doi: 10.1002/cpcb.2.