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Kuo YM , Henry RA , Andrews AJ
Measuring specificity in multi-substrate/product systems as a tool to investigate selectivity in vivo
Biochim Biophys Acta. 2016 Jan;1864(1) :70-6
PMID: 26321598 PMCID: PMC4924809 URL: http://www.ncbi.nlm.nih.gov/pubmed/26321598
AbstractMultiple substrate enzymes present a particular challenge when it comes to understanding their activity in a complex system. Although a single target may be easy to model, it does not always present an accurate representation of what that enzyme will do in the presence of multiple substrates simultaneously. Therefore, there is a need to find better ways to both study these enzymes in complicated systems, as well as accurately describe the interactions through kinetic parameters. This review looks at different methods for studying multiple substrate enzymes, as well as explores options on how to most accurately describe an enzyme's activity within these multi-substrate systems. Identifying and defining this enzymatic activity should help clear the way to using in vitro systems to accurately predicting the behavior of multi-substrate enzymes in vivo. This article is part of a Special Issue entitled: Physiological Enzymology and Protein Functions.
NotesKuo, Yin-Ming Henry, Ryan A Andrews, Andrew J eng Review Netherlands 2015/09/01 06:00 Biochim Biophys Acta. 2016 Jan;1864(1):70-6. doi: 10.1016/j.bbapap.2015.08.011. Epub 2015 Aug 29.