FCCC LOGO Faculty Publications
Xu G , Wang R , Wang Z , Lei Q , Yu Z , Liu C , Li P , Yang Z , Cheng X , Li G , Wu M
NGL-2 Is a New Partner of PAR Complex in Axon Differentiation
J Neurosci. 2015 May 6;35(18) :7153-64
PMID: 25948265    PMCID: PMC6605266    URL: https://www.ncbi.nlm.nih.gov/pubmed/25948265
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Neuronal polarization is pivotal for neural network formation during brain development. Axon differentiation is a hallmark of initial neuronal polarization. Here, we report that the leucine-rich repeat-containing protein netrin-G ligand-2 (NGL-2) as a polarity regulator that localizes asymmetrically in rat hippocampal neurons and is required for differentiation of the future axon. NGL-2 was associated with PAR complex, and this interaction resulted in local stabilization of axonal microtubules. Further study showed that the C terminal of NGL-2 binds to the PDZ domain of PAR6, and NGL-2 interacts with PAR3 and atypical PKCzeta (aPKCzeta), with PAR6 acting as a bridge or modifier. Then, NGL-2 regulates the local stabilization of microtubules and promotes axon differentiation by the aPKCzeta/microtubule affinity-regulating kinase 2 pathway. These findings reveal the critical role of NGL-2 in regulating axon differentiation in rat hippocampal neurons and reveal a novel partner of the PAR complex.
Xu, Gang Wang, Rong Wang, Zeyou Lei, Qianqian Yu, Zhibin Liu, Changhong Li, Peiyao Yang, Zengjie Cheng, Xiping Li, Guiyuan Wu, Minghua United States J Neurosci. 2015 May 6;35(18):7153-64. doi: 10.1523/JNEUROSCI.4726-14.2015.