FCCC LOGO Faculty Publications
Xu G , Wang R , Wang Z , Lei Q , Yu Z , Liu C , Li P , Yang Z , Cheng X , Li G , Wu M
NGL-2 Is a New Partner of PAR Complex in Axon Differentiation
J Neurosci. 2015 May 6;35(18) :7153-64
PMID: 25948265    PMCID: PMC6605266    URL: https://www.ncbi.nlm.nih.gov/pubmed/25948265
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Abstract
Neuronal polarization is pivotal for neural network formation during brain development. Axon differentiation is a hallmark of initial neuronal polarization. Here, we report that the leucine-rich repeat-containing protein netrin-G ligand-2 (NGL-2) as a polarity regulator that localizes asymmetrically in rat hippocampal neurons and is required for differentiation of the future axon. NGL-2 was associated with PAR complex, and this interaction resulted in local stabilization of axonal microtubules. Further study showed that the C terminal of NGL-2 binds to the PDZ domain of PAR6, and NGL-2 interacts with PAR3 and atypical PKCzeta (aPKCzeta), with PAR6 acting as a bridge or modifier. Then, NGL-2 regulates the local stabilization of microtubules and promotes axon differentiation by the aPKCzeta/microtubule affinity-regulating kinase 2 pathway. These findings reveal the critical role of NGL-2 in regulating axon differentiation in rat hippocampal neurons and reveal a novel partner of the PAR complex.
Notes
Xu, Gang Wang, Rong Wang, Zeyou Lei, Qianqian Yu, Zhibin Liu, Changhong Li, Peiyao Yang, Zengjie Cheng, Xiping Li, Guiyuan Wu, Minghua United States J Neurosci. 2015 May 6;35(18):7153-64. doi: 10.1523/JNEUROSCI.4726-14.2015.