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Pogash TJ , El-Bayoumy K , Amin S , Gowda K , de Cicco RL , Barton M , Su Y , Russo IH , Himmelberger JA , Slifker M , Manni A , Russo J
Oxidized derivative of docosahexaenoic acid preferentially inhibit cell proliferation in triple negative over luminal breast cancer cells
In Vitro Cell Dev Biol Anim. 2015 Feb;51(2) :121-7
PMID: 25413005    PMCID: PMC4948656    URL: https://www.ncbi.nlm.nih.gov/pubmed/25413005
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Omega-3 polyunsaturated fatty acids (PUFAs) exert an anticancer effect by affecting multiple cellular mechanisms leading to inhibition of proliferation and induction of apoptosis. It is well known that breast cancer comprises distinct molecular subtypes which differ in their responsiveness to therapeutic and preventive agents. We tested the hypothesis that n-3FA may preferentially affect triple-negative breast cancer cells for which no targeted intervention is presently available. The in vitro antiproliferative effects of n-3 PUFA docosahexaenoic acid (DHA) and its metabolite, 4-OH-DHA as well as its putative metabolite 4-OXO-DHA, were tested in five triple-negative human basal breast cell lines at different stages of transformation (MCF-10F, trMCF, bsMCF, MDA-MB-231, and BT-549) and three luminal breast cancer cell lines (MCF-7, T-47D, and SK-BR-3). Cell proliferation was measured with the tetrazolium MTT (3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide) assay. DHA and its oxidized derivatives significantly inhibited cell proliferation (20-90% reduction) of both basal and luminal breast cancer cell lines. The inhibitory effect was more pronounced on triple-negative basal breast cancer cell lines as compared to luminal breast cancer cell lines after 4-OXO-DHA treatment. Our data provide novel information regarding the preferential antitumor effect of oxidized derivatives of DHA on basal type breast cancer.
Pogash, Thomas J El-Bayoumy, Karam Amin, Shantu Gowda, Krishne de Cicco, Ricardo Lopez Barton, Maria Su, Yanrong Russo, Irma H Himmelberger, Julie A Slifker, Michael Manni, Andrea Russo, Jose eng P30 CA006927/CA/NCI NIH HHS/ CA06927/CA/NCI NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Germany In Vitro Cell Dev Biol Anim. 2015 Feb;51(2):121-7. doi: 10.1007/s11626-014-9822-6. Epub 2014 Nov 21.