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Poleshko A , Katz RA
Specifying peripheral heterochromatin during nuclear lamina reassembly
Nucleus. 2014 Jan-Feb;5(1) :32-9
PMID: 24637393    PMCID: PMC4028353    URL: https://www.ncbi.nlm.nih.gov/pubmed/24637393
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A conserved organizational feature of eukaryotic nuclei is the peripheral heterochromatin compartment, which provides a protected area for epigenetically silent genes and gene-poor DNA. In metazoan cells this compartment is associated with the nuclear lamina, the protein meshwork at the inner edge of the nucleus. Heterochromatin-nuclear lamina interactions promote epigenetic gene silencing, which may drive many normal and diseased biological processes. We recently obtained evidence that a previously unstudied human protein, PRR14, participates in the tethering of heterochromatin to the inner nuclear periphery. PRR14 associates with the nuclear lamina and attaches to heterochromatin through its binding partner, heterochromatin protein 1 (HP1). After disassembly early in mitosis, PRR14 reassembles in two steps, first binding to anaphase chromosomes through HP1, followed by association with the nuclear lamina in telophase. PRR14 may thereby play a role in specifying HP1-bound heterochromatin for reattachment to the nuclear lamina at mitotic exit. Here we review the relevant literature, summarize our initial work, and provide additional comments and findings.
Poleshko, Andrey Katz, Richard A eng P30 CA006927/CA/NCI NIH HHS/ R01 CA071515/CA/NCI NIH HHS/ R01 DK082498/DK/NIDDK NIH HHS/ Review Nucleus. 2014 Jan-Feb;5(1):32-9. doi: 10.4161/nucl.28167. Epub 2014 Feb 10.