This is an archive of papers published by the staff and faculty of Fox Chase Cancer Center. For questions about content, please contact Talbot Research Library
Last updated on
Li CW , Negendank WG , MurphyBoesch J , PadavicShaller K , Brown TR
Molar quantitation of hepatic metabolites in vivo in proton- decoupled, nuclear Overhauser effect enhanced P-31 NMR spectra localized by three-dimensional chemical shift imaging
Nmr in Biomedicine. 1996 Jun;9(4) :141-155
AbstractProton decoupling and nuclear Overhauser effect (NOE) enhancement significantly improve the signal-to-noise ratio and enhance resolution of metabolites in in vivo P-31 MRS, We obtained proton-decoupled, NOE-enhanced, phospholipid-saturated P-31 spectra localized to defined regions within the normal liver using three-dimensional chemical shift imaging, Proton- decoupling resulted in the resolution of two major peaks in the phosphomonoester (PME) region, three peaks in the phosphodiester (PDE) region and a diphosphodiester peak, In order to obtain molar quantitation, we measured the NOE of all hepatic phosphorus resonances, and we corrected for saturation effects by measuring hepatic metabolite T-1 using the variable nutation angle method with phase-cycled, B-1-independent rotation, adiabatic pulses, After corrections for saturation effects, NOE enhancement, B, variations and point spread effects, the following mean concentrations (mmol/l of liver) (+/-SD) were obtained: [PME(1)]=1.2+/-0.4, [PME(2)+2,3- DPG]=1.1+/-0.1, [Pi+2,3-DPG]=2.8+/-0.5, [GPEth]=2.8+/-0.7, [GPChol]=3.5+/-0.6 and [beta-NTP]=3.8+/-0.3. T-1 and NOE enhancement were strongly correlated (r=90), and indicated that the fractional contribution of H-1-P-31 dipolar relaxation to total P-31 relaxation is minimal for NTPs, moderate for PMEs and high for PDEs in liver Proton-decoupling and NOE enhancement permit one to obtain more information about in vivo metabolism of liver than previously available and should enhance the utility of P-31 MRS for the study of hepatic disorders.
NotesTimes Cited: 10 English Article WD810 NMR BIOMED