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Maradeo ME , Skibbens RV
Replication Factor C Complexes Play Unique Pro- and Anti-Establishment Roles in Sister Chromatid Cohesion
PLoS One. 2010 Oct;5(10) :E15381
PMID: ISI:000283537000062    PMCID: PMCID: PMC2965161   
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Recent studies have lead to a rapid expansion of sister chromatid cohesion pathways. Of particular interest is the growth in classifications of anti-establishment factors-now including those that are cohesin-associated (Rad61/WAPL and Pds5) or DNA replication fork-associated (Elg1-RFC). In this study, we show that the two classes of anti-establishment complexes are indistinguishable when challenged both genetically and functionally. These findings suggest that both classes function in a singular pathway that is centered on Ctf7/Eco1 (herein termed Ctf7) regulation. The anti-establishment activity of Elg1-RFC complex is particular intriguing given that an alternate Ctf18-RFC complex exhibits robust pro-establishment activity. Here, we provide several lines of evidence, including the use of Ctf7 bypass suppressors, indicating that these activities are not simply antagonistic. Moreover, the results suggest that Ctf18-RFC is capable of promoting sister chromatid pairing reactions independent of Ctf7. The combination of these studies suggest a new model of sister chromatid pairing regulation.
Maradeo, Marie E. Skibbens, Robert V. National Institutes of General Medicine [1R15GM083269]; Susan G. Komen for a Cure Foundation [BCTR0707708] RVS is supported by award 1R15GM083269 from the National Institutes of General Medicine (http://www.nih.gov) and also by award BCTR0707708 from the Susan G. Komen for a Cure Foundation (http://ww5.komen.org). Any opinions, findings, and conclusions or recommendations expressed in this study are those of the author(s) and do not necessarily reflect the views of the National Institutes of General Medicine nor those of the Susan G. Komen Foundation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. 45 Public library science; 185 berry st, ste 1300, san francisco, ca 94107 usa 671un