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Lake RJ , Grimm LM , Veraksa A , Banos A , Artavanis-Tsakonas S
In Vivo Analysis of the Notch Receptor S1 Cleavage
PLoS One. 2009 Aug;4(8 ) :e6728
PMID: ISI:000269268300008    PMCID: PMC2726433   
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A ligand-independent cleavage (S1) in the extracellular domain of the mammalian Notch receptor results in what is considered to be the canonical heterodimeric form of Notch on the cell surface. The in vivo consequences and significance of this cleavage on Drosophila Notch signaling remain unclear and contradictory. We determined the cleavage site in Drosophila and examined its in vivo function by a transgenic analysis of receptors that cannot be cleaved. Our results demonstrate a correlation between loss of cleavage and loss of in vivo function of the Notch receptor, supporting the notion that S1 cleavage is an in vivo mechanism of Notch signal control.
Lake, Robert J. Grimm, Lisa M. Veraksa, Alexey Banos, Andrew Artavanis-Tsakonas, Spyros 31 Public library science; 185 berry st, ste 1300, san francisco, ca 94107 usa 487jf