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Dong XY , Guo P , Boyd J , Sun XD , Li QN , Zhou W , Dong JT
Implication of snoRNA U50 in human breast cancer
Journal of Genetics and Genomics. 2009 Aug;36(8) :447-454
PMID: 19683667    PMCID: PMC2854654    URL: https://www.ncbi.nlm.nih.gov/pubmed/19683667
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Deletion of chromosome 6q is frequent in breast cancer, and the deletion often involves a region in 6q14-q16 . At present, however, the underlying tumor suppressor gene has not been established. Based on a recent study identifying snoRNA U50 as a candidate for the 6q14-16 tumor suppressor gene in prostate cancer, we investigated whether U50 is also involved in breast cancer. PCR-based approaches showed that U50 underwent frequent genomic deletion and transcriptional downregulation in cell lines derived from breast cancer. Mutation screening identified the same 2-bp deletion of U50 as in prostate cancer in both cell lines and primary tumors from breast cancer, and the deletion was both somatic and in germline. Genotyping of a cohort of breast cancer cases and controls for the Mutation demonstrated that, while homozygous genotype of the Mutation was rare, its heterozygous genotype occurred more frequently in women with breast cancer. Functionally, re-expression of U50 resulted in the inhibition of colony formation in breast cancer cell lines. These results suggest that noncoding snoRNA U50 plays a role in the development and/or progression of breast cancer.
Dong, Xue-Yuan Guo, Peng Boyd, Jeff Sun, Xiaodong Li, Qunna Zhou, Wei Dong, Jin-Tang National Cancer Institute, USA [R01 CA085560).] This work was supported in part by a grant from the National Cancer Institute, USA (No. R01 CA085560). 38 Science press; 16 donghuangchenggen north st, beijing 100717, peoples r china 483pw