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Golemis EA , Serebriiskii I , Finley RL Jr , Kolonin MG , Gyuris J , Brent R
Interaction trap/two-hybrid system to identify interacting proteins
Curr Protoc Protein Sci. 2009 Aug;Chapter 19 :Unit 19.2
AbstractThe yeast two-hybrid method (or interaction trap) is a powerful technique for detecting protein interactions. The procedure is performed using transcriptional activation of a dual reporter system in yeast to identify interactions between a protein of interest (the bait protein) and the candidate proteins for interaction. The method can be used to screen a protein library for interactions with a bait protein or to test for association between proteins that are expected to interact based on prior evidence. Interaction mating facilitates the screening of a library with multiple bait proteins.
NotesGolemis, Erica A Serebriiskii, Ilya Finley, Russell L Jr Kolonin, Mikhail G Gyuris, Jeno Brent, Roger United States Current protocols in protein science / editorial board, John E. Coligan ... [et al.] Curr Protoc Protein Sci. 2009 Aug;Chapter 19:Unit19.2.