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Wambi CO , Sanzari JK , Sayers CM , Nuth M , Zhou ZZ , Davis J , Finnberg N , Lewis-Wambi JS , Ware JH , El-Deiry WS , Kennedy AR
Protective Effects of Dietary Antioxidants on Proton Total-Body Irradiation-Mediated Hematopoietic Cell and Animal Survival
Radiation Research. 2009 Aug;172(2) :175-186
PMCID: PMC 2746923   
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Abstract
Dietary antioxidants have radioprotective effects after gamma-radiation exposure that limit hematopoietic cell depletion and improve animal survival. The purpose of this study was to determine whether a dietary Supplement consisting of L-selenomethionine, vitamin C, vitamin E succinate, a-lipoic acid and N-acetyl cysteine could improve survival of mice after proton total-body irradiation (TBI). Antioxidants significantly increased 30-day survival of mice only when given after irradiation at a dose less than the calculated LD50/30; for these data, the dose-modifying factor (DMF) was 1.6. Pretreatment of animals with antioxidants resulted in significantly higher serum total white blood cell, polymorphonuclear cell and lymphocyte cell counts at 4 h after 1 Gy but not 7.2 Gy proton TBI. Antioxidants significantly modulated plasma levels of the hematopoietic cytokines Flt-3L and TGF beta 1 and increased bone marrow cell counts and spleen mass after TBI. Maintenance of the antioxidant diet resulted in improved recovery of peripheral leukocytes; and platelets after sublethal and potentially lethal TBI. Taken together, oral supplementation with antioxidants appears to be an effective approach for radioprotection of hematopoietic cells and improvement of animal survival after proton TBI. (C) 2009 by Radiation Research Society
Notes
Wambi, Chris O. Sanzari, Jenine K. Sayers, Carly M. Nuth, Manunya Zhou, Zhaozong Davis, James Finnberg, Niklas Lewis-Wambi, Joan S. Ware, Jeffrey H. El-Deiry, Wafik S. Kennedy, Ann R. Radiation research soc Lawrence 478wy